PrInSeS

Primer-initiated de novo sequence synthesis

PrInSeS

We present PrInSeS, a software tool enabling de novo sequence assembly from short reference sequence seeds using high-throughput sequencing data. We demonstrate PrInSeS’ utility in clone sequence verification, cDNA library screening, and genome sequence assembly and evaluate its performance in characterizing sequence variation using simulated and experimental data, and by method comparison. PrInSeS should be of great value for anyone interested in low-cost, high-throughput sequence identification, annotation, and assembly.

Publication:

Primer-initiated sequence synthesis to detect and assemble structural variants

Andreas Massouras, Korneel Hens, Carine Gubelmann, Swapna Uplekar, Frederik Decouttere, Jacques Rougemont, Stewart T Cole & Bart Deplancke

If you use PrInSeS-G, please also join our Google group http://groups.google.com/group/prinsesg for updates and discussion.

All software is freely available [download here]